Scaffold plays a key role in cell proliferation in a three-­dimensìonal cell culture. Collagen, a well-known scaffold, is widely used in this field. However, its animal­-derived nature and variability may contribute to variations in experimental data.

To solve this problem, We developed PanaceaGel as a superior 3D cell culture alternative to Collagen.PanaceaGel does not include any animal-derived ingredients. instead, PanaceaGel is composed of 100% synthetic peptides, which self-assemble to form a nanofiber-scale three-dimensional network in aqueous solvent.

The main component of PanaceaGel is a thirteen amino acid synthetic peptide, which is made of four naturally occurring amino acids in a specific sequence (US 2012/0058066 A1). The primary structure is:

The thirteen amino acid peptides undergo spontaneous self-assembly in several steps to form nanotibers in aqueous solvents due to hydrogen bonding, electrostatic interaction and hydrophobic interaction. These nanofibers assemble into a higher-order structure, a nanofiber-scale three-dimensional network, which forms a transparent gel with a moisture content of over 99%.

PanaceaGel is a 100% synthetic peptide hydrogel with a neutral pH range. It could be used as a scaffold in three-dimensional cell culture and as a carrier for drug delivery. lt does not contain any animal-derived or biologically active components, thus increasing experimental reproducibility compared to Collagen-based 3D cell cultures.

The main component of PanaceaGel is a thirteen amino acid peptide, composed of four types of naturally occurring amino acids, arginine, leucine, aspartic acid and alanine.
The peptides self-assemble into a three-dimensional network to form a hydrogel in aqueous solvent.

PanaceaGel is in a neutral pH range. Therefore, no pre-treatment is necessary before being used with a cell suspension.

Three-dimensional cell culture scaffold, drug delivery carrier, tissue engineering, and more.

Three-dimensional cell culture in PanaceaGel
A. Mouse skeletal muscle cell (C2C12) proliferation (day 8). Blue: DAPI staining,
Green: Calcein staining. Scale bar = 50μm.
B. Human chondrosarcoma cell (OUMS-27) immunostaining (day 12).
Blue: DAPI staining, Green: agrecan, Red: Type II collagen. Scale bar = 10μm.

PanaceaGel has been successfully used in the three-dimensional culture of mouse skeletal muscle cell (C2C12, see image A), human chondrosarcoma cell (OUMS-27, see image B), rat skeletal muscle cell (L6), isolated rat neonatal cardiac myocyte, and human fetal lung fibroblast (TIG-l ).

＜Reference＞
1_ Nagai Y, Yokoi H, Kaihara K, Naruse K. The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel. Biomaterials 2012;33(4):1044-1051

• ・100% synthetic peptide gel scaffold
• ・No animal-derived components or pathogens
• ・Transparent and neutral pH range
• ・Ready to use
• ・Isotonic options available (SPG-178-204 and SPG-178-208)
• ・Store at room temperature

＜Comparison Sheet＞

＜Product Specifications＞