Scaffold plays a key role in cell proliferation in a three-dimensìonal cell culture. Collagen, a well-known scaffold, is widely used in this field. However, its animal-derived nature and variability may contribute to variations in experimental data.
To solve this problem, We developed PanaceaGel as a superior 3D cell culture alternative to Collagen.PanaceaGel does not include any animal-derived ingredients. instead, PanaceaGel is composed of 100% synthetic peptides, which self-assemble to form a nanofiber-scale three-dimensional network in aqueous solvent.
The main component of PanaceaGel is a thirteen amino acid synthetic peptide, which is made of four naturally occurring amino acids in a specific sequence (US 2012/0058066 A1). The primary structure is:
The thirteen amino acid peptides undergo spontaneous self-assembly in several steps to form nanotibers in aqueous solvents due to hydrogen bonding, electrostatic interaction and hydrophobic interaction. These nanofibers assemble into a higher-order structure, a nanofiber-scale three-dimensional network, which forms a transparent gel with a moisture content of over 99%.
ßsheet structure Thirteen amino acid peptides form ß sheets due to hydrogen bonding, electrostatic interactions and hydrophobic interactions. |
Nanofiber formation Each β sheet has two sides: a hydrophilic surface and a hydrophobic surface. β sheets stack to each other to form nanofibers in aqueous solvent due to hydrophobic interactions. |
Nanofiber network structure Nanofibers interact with each other to form a network containing more than 99% water. |
PanaceaGel is a 100% synthetic peptide hydrogel with a neutral pH range. It could be used as a scaffold in three-dimensional cell culture and as a carrier for drug delivery. lt does not contain any animal-derived or biologically active components, thus increasing experimental reproducibility compared to Collagen-based 3D cell cultures.
The main component of PanaceaGel is a thirteen amino acid peptide, composed of four types of naturally occurring amino acids, arginine, leucine, aspartic acid and alanine.
The peptides self-assemble into a three-dimensional network to form a hydrogel in aqueous solvent.
PanaceaGel is in a neutral pH range. Therefore, no pre-treatment is necessary before being used with a cell suspension.
Three-dimensional cell culture scaffold, drug delivery carrier, tissue engineering, and more.
PanaceaGel has been successfully used in the three-dimensional culture of mouse skeletal muscle cell (C2C12, see image A), human chondrosarcoma cell (OUMS-27, see image B), rat skeletal muscle cell (L6), isolated rat neonatal cardiac myocyte, and human fetal lung fibroblast (TIG-l ).
<Reference>
1_ Nagai Y, Yokoi H, Kaihara K, Naruse K. The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel. Biomaterials 2012;33(4):1044-1051
<Comparison Sheet>
PanaceaGel | Collagen | Biodegradable polymer | |
---|---|---|---|
Origin | Synthetic | Animal-derived | Synthetic |
Stiffness | Middle | Middle | Hard |
pH | Neutral | Neutral・Acidic | Neutral |
Biocompatibility | Good | Excellent | Good |
Transparency | High | High | Low |
<Product Specifications>
Catalog Number | SPG-178-004 | SPG-178-204 | SPG-178-008 | SPG-178-208 |
---|---|---|---|---|
Peptide concentration |
0.4 w/w% | 0.8 w/w% | ||
pH | Neutral | |||
Isotonic Option | No | Yes | No | Yes |
Autoclaved | Yes | |||
Volume | 1mL/2mL |